Coding

Part:BBa_K1702001:Design

Designed by: Anthony Roulier, Chauncy Hinshaw, Krista Henderson   Group: iGEM15_CSU_Fort_Collins   (2015-09-17)


Overexpression of E. coli fadL under control of lac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1102
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This plasmid allows for induced expression of fadL with lactose or IPTG.


Source

The lac gene is sourced from the iGEM distribution kit. The fadL gene was extracted by genomic PCR from DH5alpha E. coli.

References